Direct Line

Microplate Reader Application Compatibility

Microplate readers by Berthold Technologies are available for fluorescence, absorbance, luminescence, TRF, HTRF®, fluorescence polarization, BRET and AlphaScreen®. The table below details what technologies and features are available in each instrument, and with which popular assays is each of them compatible, but it does not cover all existing assays. If you have any questions about the assays in this table, or about any other assay you may be interested on, please contact us.

Detection Modes Apollo 11
LB 913
Orion II
LB 965
CentroPRO
LB 962
Centro
LB 960
Centro XS³
LB 960
Tristar²
LB 942
Tristar² S
LB 942
Mithras
LB 940
Mithras²
LB 943
Absorbance
+




+
+
+
+
Fluorescence





+
+
+
+
Fluorescence with injectors





+
+
+
+
FRET





+
+
+
+
Fluorescence polarization (FP)






+
+
+
Time-resolved fluorescence (TRF)






+
+
+
HTRF®





+
+
+
AlphaScreen®






+
+
Glow Luminescence
+
+
+
+
+
+
+
+
Flash Luminescence (with injectors)

+

+
+
+
+
+

BRET




+
+
+
+
Technical Features Apollo 11
LB 913
Orion II
LB 965
CentroPRO
LB 962
Centro
LB 960
Centro XS³
LB 960
Tristar²
LB 942
Tristar² S
LB 942
Mithras
LB 940
Mithras²
LB 943
Bottom reading + + +
Monochromator for Absorbance and Excitation + +
Monochromator for Emission +
Injectors up to 4 up to 3 up to 3 up to 3 up to 3 up to 4 up to 4
Temperature control + + + + + + +
Shaking + + + + +
Cooled PMT + + + +
Gas connection + + +
Absorbance spectral scanning + +
Fluorescence spectral scanning + +
Luminescence spectral scanning + +
96 well + + + + + + + + +
384 well + + + + + + +
6-384 well + + + +
6-1536 well + +
Assay Types Apollo 11
LB 913
Orion II
LB 965
CentroPRO
LB 962
Centro
LB 960
Centro XS³
LB 960
Tristar²
LB 942
Tristar² S
LB 942
Mithras
LB 940
Mithras²
LB 943
Endpoint assays + + + + + + + + +
Kinetic assays + + + + + + +
Well scanning + + + + + +
ELISA (absorbance) + + + + +
Fluorescence immunoassays + + + +
Luminescence immunoassays + + + + + + +
Immunoassays + + + + + + + + +
Protein Quantification Apollo 11
LB 913
Orion II
LB 965
CentroPRO
LB 962
Centro
LB 960
Centro XS³
LB 960
Tristar²
LB 942
Tristar² S
LB 942
Mithras
LB 940
Mithras²
LB 943
Bradford, BCA, Lowry + + + + +
Protein (A280) + + + +
Low volume absorbance (2 µL) +
NanoOrange®, CBQCA, Quant-iT™ + + + +
Nucleic Acid Quantification Apollo 11
LB 913
Orion II
LB 965
CentroPRO
LB 962
Centro
LB 960
Centro XS³
LB 960
Tristar²
LB 942
Tristar² S
LB 942
Mithras
LB 940
Mithras²
LB 943
DNA, RNA, oligos (A260) + + + +
A260/A280 + + + +
Low volume DNA samples (2 µL) +
RiboGreen®, PicoGreen®, Hoechst, OliGreen® + + + +
Reporter Genes Apollo 11
LB 913
Orion II
LB 965
CentroPRO
LB 962
Centro
LB 960
Centro XS³
LB 960
Tristar²
LB 942
Tristar² S
LB 942
Mithras
LB 940
Mithras²
LB 943
Secreted alkaline phosphatase (SEAP) + + + + + + + + +
ß-Galactosidase (GUS) + + + + + + + +
ß-Glucuronidase (ß-Gal) + + + + + + + + +
Luciferase + + + + + + + +
Dual Luciferase + + + + + + + +
GFP, CFP, YFP + + + +
Cell Viability, Proliferation and Death Apollo 11
LB 913
Orion II
LB 965
CentroPRO
LB 962
Centro
LB 960
Centro XS³
LB 960
Tristar²
LB 942
Tristar² S
LB 942
Mithras
LB 940
Mithras²
LB 943
MTT, XTT, Crystal Violet, SRB + + + + +
Fluorescent Live/Dead kits, Alamar Blue + + + +
Calcein AM, CyQUANT® + + + +
SYTOX® Green, ethidium homodimer + + + +
Enzyme Assays Apollo 11
LB 913
Orion II
LB 965
CentroPRO
LB 962
Centro
LB 960
Centro XS³
LB 960
Tristar²
LB 942
Tristar² S
LB 942
Mithras
LB 940
Mithras²
LB 943
ATP (luciferase) + + + + + + + +
NADH (dehydrogenases) + + + + + + + + +
Phosphatases/Kinases activity + + + + + + + +
Protease activity + + + + + + + + +
HTS Assays Apollo 11
LB 913
Orion II
LB 965
CentroPRO
LB 962
Centro
LB 960
Centro XS³
LB 960
Tristar²
LB 942
Tristar² S
LB 942
Mithras
LB 940
Mithras²
LB 943
HTRF® (Cisbio) + + +
AlphaScreen® (Perkin Elmer) + +
DELFIA®(Perkin Elmer) + + +
LANCE® (Perkin Elmer) + + +
LanthaScreen® (Invitrogen) + + +
PolarScreen™ (Invitrogen) + + +
Transcreener® ADP² FP (BellBrook Labs) + + +
Z'-Lyte (Invitrogen) + + + +
Ion Channel - VSP (Invitrogen) + + + +
P450-Glo™ (Promega) + + + + + + + +
DLR™ (Promega) + + + + + + +
Dual-Glo™ (Promega) + + + + + + + +
HitHunter® (DiscoveRx, luminescence) + + + + + + + +
HitHunter® (DiscoveRx, FP) + + +
MycoAlert™ (Cambrex) + + + + + + + +
PKLight® (Cambrex) + + + + + + + +
ATP bioluminescence + + + + + + + +
Intracellular calcium (Ca2+ assay) + + + + + + +
Luminescent ABEL® antioxidant test + + + + + + + +

Chroma-Glo, Kinase-Glo, DLR, Dual-Glo and P450-Glo are trademarks of Promega Corp. MycoAlert and PK Light are trademarks of Cambrex. HitHunter is a trademark of DiscoveRx. Adapta, NanoOrange, PicoGreen, RiboGreen, OliGreen, Quant-iT, CBQCA, PolarScreen, LanthaScreen, Ion Channel VSP, Z-Lyte, Alamar Blue, and Alexa Fluor are trademarks or registered trademarks of Invitogen Corp. AlphaScreen, AlphaLISA, AlphaPlex, DELFIA, and LANCE are registered trademarks of Perkin Elmer Inc. HTRF is a registered trademark of Cisbio International. Transcreener is a patented technology of BellBrook Labs. Cy Dyes are trademarks of GE Healthcare.