Luminescence Microplate Readers
Luminescence does not require a light source for excitation but is the result of a chemical or biochemical reaction. Consequently, the optical system of a typical luminometer consists of two key components: a light-tight chamber to read the signal and a PMT to detect it. In general, two different types of PMTs are getting used, current mode PMTs and photon-counting PMTs. The latter are widely accepted as being the most sensitive devices for detecting luminescence and are the heart of all luminescence detection systems developed by Berthold Technologies.
Our Luminescence plate readers have been brought to market by a team with a heritage of developing the most sensitive Luminometers. All high-performance plate luminometers “Made in Germany” by Berthold Technologies guarantee lowest background and negligible crosstalk. We offer both dedicated plate luminometers and multimode plate readers configured to perform luminescence detection.
Dedicated Microplate Luminometers
Both microplate and tube luminometers are suitable for most applications, including reporter genes, luminescent immunoassays, caspase and kinase assays and others, but some applications may involve large amounts and samples, and in this case microplate luminometers are the instrument of choice.
For example, ATP determination is a popular method for HTS (High Throughput Screening). ATP is present in all living cells and is needed in the reaction of luciferin with luciferase to produce light, so it is not surprising that luminescence-based ATP assays are often used to quantify living cells, for example, to asses cell viability, cell proliferation or biomass.
Microplate luminescence readers are the instrument of choice for BRET (Bioluminescence Resonance Energy Transfer), as the instrument must feature sequential dual luminescence measurements at different wavelengths: one for the donor and one for the acceptor for each microplate well, and this feature is frequently absent in dedicated luminometers. BRET is based on the fact that the energy derived from a luciferase reaction can be used to excite a fluorescent protein if the latter is in close proximity to the luciferase enzyme.
If you would like to automate your chemiluminescence immunoassays you may be interested on our Assay Workstations.