Absorbance Microplate Readers - Berthold Technologies GmbH & Co.KG

ELISA Reader Apollo

The ELISA Reader Apollo combines measurement of 96-well plates in 6 s with intuitive 7-inch touchscreen operation for all your ELISA assays.

Tristar 3 Multimode Reader

The Tristar 3 is a user-friendly and affordable filter-based multimode plate reader that offers high-performance analysis in absorbance, luminescence and fluorescence measurements

Tristar 5 Multimode Reader

The Tristar 5 is a modular high-performance microplate reader equipped with independent, user-selectable filters and monochromators on both, the excitation and emission side for any measurement

Compare Absorbance Microplate Readers

	Apollo	Tristar 3	Tristar 5
Detectors	Photodiode for absorbance	Low-noise photomultiplier tube in dual mode Photodiode for absorbance	2 Low-noise photomultiplier tube in dual mode Photodiode for absorbance
Absorbance (Vis)
Absorbance (UV)
Luminescence
Fluorescence
TRF
FP
AlphaScreen

	Apollo	Tristar 3	Tristar 5
Optical system	Single technology	Single path for all detection technologies	Single path for all detection technologies
Light source	Quartz Halogen Lamp	Xenon flash lamp	Xenon flash lamp Laser diode (680 nm)
Spectral range Absorbance	340-750 nm	200-1000 nm	200-1000 nm
Z-adjustment
Bottom reading
Filters
Monochromator for Abs. and Fluor. Excitation
Monochromator for Fluor. Emission
Filter identification	Manual	Manual	Manual
Wavelength scanning (absorbance)		✘
Monochromator stray light			10^-6
Monochromator bandwidth			4-22 nm

	Apollo	Tristar 3	Tristar 5
Injectors	No	Up to 3 JET injectors with frictionless operation	Up to 3 JET injectors with frictionless operation
Injector volume		10-100 µL in 1 µL steps	10-100 µL in 1 µL steps
Injector accuracy		Better than 2% across the entire volume range	Better than 2% across the entire volume range
Injector precision		Better than 2% across the entire volume range	Better than 2% across the entire volume range

	Apollo	Tristar 3	Tristar 5
Microplate formats	96-well	6- to 384-well	6- to 384-well
Additional sample formats (optional)		µDrop plate for microvolumes Adapter for cuvettes Adapter for petri dishes Adapter for Terasaki plates	µDrop plate for microvolumes Adapter for cuvettes Adapter for petri dishes Adapter for Terasaki plates
Optional Microplate Stacker

	Apollo	Tristar 3	Tristar 5
Shaking	3 shaking modes: slow, medium and fast	Linear, orbital, double orbital, with selectable speed and diameter	Linear, orbital, double orbital, with selectable speed and diameter
Temperature control	from ambient+ 4° C to 50° C (Apollo-I only)	From ambient+5° to 45° C	From ambient+5° to 45° C
Gas connection

	Apollo	Tristar 3	Tristar 5
Absorbance precision	≤ 0.2 % CV (@0-3 OD) (@405 nm) ≤ 1 % CV (@3-4 OD) (@405 nm)	Better than 0.6%	Better than 0.6%
Absorbance accuracy	± 1 % or ± 0.005 OD (0-3 OD) whichever is greater (@405 nm) ± 2 % (3-4 OD) (@405 nm)	Better than 2%	Better than 2%
Dynamic range	0-4 OD	0-3.5 OD	0-3.5 OD

Apollo

Tristar 3

Tristar 5

Absorbance precision

≤ 0.2 % CV (@0-3 OD) (@405 nm)

≤ 1 % CV (@3-4 OD) (@405 nm)

Better than 0.6%

Absorbance accuracy

± 1 % or ± 0.005 OD (0-3 OD) whichever is greater (@405 nm)

± 2 % (3-4 OD) (@405 nm)

Better than 2%

Dynamic range